Quinoprotein Ethanol Dehydrogenase:Preparation of the Apo-form and Reconstitution with Pyrroloquinoline Quinone and Ca2+ or Sr2+ Ions.
نویسندگان
چکیده
منابع مشابه
Structure of the pyrroloquinoline quinone radical in quinoprotein ethanol dehydrogenase.
Quinoprotein alcohol dehydrogenases use the pyrroloquinoline quinone (PQQ) cofactor to catalyze the oxidation of alcohols. The catalytic cycle is thought to involve a hydride transfer from the alcohol to the oxidized PQQ, resulting in the generation of aldehyde and reduced PQQ. Reoxidation of the cofactor by cytochrome proceeds in two sequential steps via the PQQ radical. We have used a combina...
متن کاملThe pyrroloquinoline quinone (PQQ)-containing quinoprotein dehydrogenases.
L. (1995) Biochemistry 34, 11080-1 1089 10 Friden, H. and Hederstedt, L. (1990) Mol. Microbiol. 4, 1045-1056 1 1 Nakamura, K., Yamaki, M., Sarada, M., Nakayama, S., Vibat, C. R. T., Gennis, R. B., Nakayashiki, T., Inokuchi, H., Kojima, S. and Kita, K. (1996) J. Biol. Chem. 271, 521-527 12 Hagerhall, C. and Hederstedt, L. (1996) FEBS Lett. 389, 25-31 13 Pennoyer, J. D., Ohnishi, T. and Trumpower...
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The reconstitution of active holoenzyme containing calcium from inactive calcium-free methanol dehydrogenase, isolated from a moxA mutant of Methylobacterium extorquens, has a pH optimum of about pH 10, with a well defined pK for the process at pH 9.3. Two Ca2+ ions were irreversibly incorporated per alpha 2 beta 2 tetramer. Calcium could be replaced in the incorporation process by strontium or...
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The soluble form of the homogeneous quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus is reversibly inactivated at temperatures above 35 degrees C. An equilibrium is established between active and denatured enzyme, this depending on the protein concentration and the inactivation temperature used. Upon thermal inactivation the enzyme dissociates into the prosthetic group pyrrol...
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abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1991
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.55.1721